Frank Lampard’s Rams, currently outside of the Championship play-off places on goal difference.
Beat struggling Southampton on penalties in a third-round replay before seeing off the spirited challenge of third-tier Accrington Stanley courtesy of Martyn Waghorn’s late strike.
They may fancy their chances of springing another surprise on the south coast and reaching the quarter-finals for the first time since 1999.
With Brighton having won just one and lost seven of their last 11 Premier League games, a run which sees them now sit just three points above the relegation zone.
They began their latest FA Cup campaign with a comfortable 3-1 triumph at Bournemouth before needing two attempts to oust West Brom.
Glenn Murray was the hero at The Hawthorns, scoring twice in extra-time to seal a 3-1 replay victory.
Solly March is Brighton’s only major injury doubt for the FA Cup fifth-round tie at home to Derby on Saturday (kick-off 12.30pm).
The winger will be assessed on the ankle problem which forced him off in last Saturday’s Premier League defeat to Burnley.
Albion boss Chris Hughton revealed a few unnamed players are suffering with knocks.
Team News of Brighton vs Derby
Brighton could be boosted by the return of winger Jose Izquierdo, who is back in full training this week after recovering from a knee injury.
However, Solly March (ankle) is doubtful after limping out of last weekend’s 3-1 home defeat by resurgent Burnley.
Striker Florin Andone will serve the second game of a three-match suspension handed down retrospectively after an FA charge of violent conduct that related to an apparent elbow on West Brom’s Sam Field.
As for Derby, Leicester loanee Andy King is cup-tied, but Ashley Cole could make his first start for the club.
Marcus Olsson, Craig Forsyth (both knee) and Curtis Davies (achilles) are all long-term absentees.
Whill Scott Carson and Mason Mount both missed the midweek draw at Ipswich.
Score prediction: Brighton 2-1 Derby
TV channel and live stream online coverage
The match will be televised live on BT Sport 2 and BT Sport 4K UHD. BT Sport subscribers will be able to stream the match online via the video player and the BT Sport app.
YOU CAN FOLLOW MATCH HERE
These two sides have met on 42 previous occasions, with Derby claiming 20 wins to Brighton’s 15. There have been seven draws.
They last went head to head in May 2017, when promotion chasers Brighton won 3-0 at the Amex courtesy of goals from Anthony Knockaert, Sam Baldock and Murray to move level on points with leaders Newcastle.